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Opportunity at National Institute of Standards and Technology (NIST)

Mapping Reponses in Complex Microbial Systems using Advanced Imaging

Location

Material Measurement Laboratory, Biosystems and Biomaterials Division

RO# Location
50.64.41.B8452 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

name email phone
Camp, Charles Henry charles.camp@nist.gov 301.975.4631
Dunkers, Joy P. joy.dunkers@nist.gov 301.975.6841
Lee, Young Jong youngjong.lee@nist.gov 301.975.6832

Description

Quantifying composition and function of complex microbial systems is key to progress in understanding the important role that bacteria play in human health, from the gut microbiome to the global problem of anti-microbial resistance. Traditional microbial characterization approaches are often destructive, insufficient in resolution and sensitivity, and are subject to bias inherent in the measurement process. New methods for quantitative, in situ characterization are essential for gaining insight into microbial communities in their unperturbed state.

This project will use in situ advanced imaging techniques such as broadband coherent anti-Stokes Raman (BCARS), quantum cascade laser infrared (QCL-IR), and fluorescence microscopies to obtain quantitative characterization of bacteria identity and location, metabolic state, and signaling molecules in commercially and clinically relevant mixed microbial communities and biofilms and compare the findings to traditional microbial analytical tools.

 

Keywords:
Bacteria; biofilm; fluorescence; imaging; infrared; microbe; microbial; microbial community; microbiology; microbiome; microscopy; Raman

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants

Stipend

Base Stipend Travel Allotment Supplementation
$74,950.00 $3,000.00
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