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Opportunity at National Institute of Standards and Technology (NIST)

Biophysics: Studying Single Biological Molecules with Atomic-Scale Resolution

Location

Physical Measurement Laboratory, Quantum Physics Division

RO# Location
50.68.92.B8346 Boulder, CO

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Perkins, Thomas Tupper tperkins@jila.colorado.edu 303.492.5291

Description

Single molecule studies are revolutionizing biophysics. The Perkins group focuses on developing and applying high-precision single-molecule studies based on optical traps and atomic force microscopes to answer fundamental biophysical questions, including: how do motor proteins transduce chemical energy into mechanical motion and what are the energetics and dynamics underling membrane proteins folding? Specifically, we developed an optical-trapping assay with Ångström-scale stability and resolution in all three dimensions and are applying these high resolution studies to DNA-based molecular motors and RNA-folding. In a parallel effort, we significantly enhanced the biophysical capabilities of an atomic force microscope (AFM). Specifically, we achieved sub-pN force precision and stability over a broad timescale (0.1-100 s) for the first time; and, most recently, improved the timescale of sub-pN precision of AFM by another factor of 50. We are currently applying these improvements to mechanically unfolding and refolding interesting biological molecules with 1-µs resolution, including membrane protein as well as continuing to pursue further metrological improvements in single molecule studies.

 

Keywords:
Atomic force microscopy; Biophysics; DNA; Membrane proteins; Microscopy; Molecular motor; Nanometer resolution; Optical trap; Optical tweezers; Single molecule; AFM;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants
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