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Opportunity at National Institute of Standards and Technology (NIST)

Molecular Biophysics and Protein Dynamics via Microfluidics and Ultrafast Spectroscopy Techniques

Location

Physical Measurement Laboratory, Quantum Physics Division

RO# Location
50.68.92.B8150 Boulder, CO

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Jimenez, Ralph rjimenez@jila.colorado.edu 303.492.8439

Description

The laboratory employs techniques from biochemistry, chemical physics, and optics. We use femtosecond laser spectroscopy to quantify the spectra of motions by probing either the electronic transitions of a bound cofactor or ligand. Experiments on site-directed mutants or chemically modified proteins are used to determine the structural origins of the motions. We are also developing and employing microfluidics-based selection strategies on combinatorial libraries of red-fluorescent proteins to improve their photophysical properties and to generate new FRET-based sensors of metals and other analytes n vivo. Finally, we are extending the integration of spectroscopy with microfluidic flow-cytometry for development of new biophotonic functionality such as photoswitchable fluorophores.

 

Keywords:
Biophysics; Fluorescence; Spectroscopy; Microfluidics;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants
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