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Opportunity at National Institute of Standards and Technology (NIST)

NMR Metabolomics to Characterize Manufacture of Therapeutic Proteins and Therapeutic Cell Lines

Location

Material Measurement Laboratory, Biomolecular Measurement Division

RO# Location
50.64.51.C0365 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Arbogast, Luke W arbogastl@ibbr.umd.edu 240.314.6270

Description

Engineered cells are central to the production of therapeutic proteins, which now account for seven of the top 10 drugs. Furthermore, revolutionary therapies such as CAR-T now make direct use of engineered cells as therapeutics. To support a thorough understanding of the relationship between cellular states during culture and the quality attributes of the resultant biologic, we aim to correlate the metabolic flux of a standard cell line to its resulting product quality attributes.  Using a Chinese Hamster Ovary (CHO) standard cell line for production of the NISTmAb reference monoclonal antibody, we aim to apply 1D and 2D NMR metabolomics to monitor growth media and cellular extracts during culture, and correlate these measurements to biologic quality attributes using chemometrics and machine learning.

References

Kashi, L.; Yandrofski, K.; Preston, R.J.; Arbogast, L.W. et. al. "Heterologous recombinant expression of a non-originator NISTmAb" mAbs, 10: 922-933, 2018.

Keywords:
Nuclear Magnetic Resonance, biotherapeutics, monoclonal antibodies, biomanufacturing, metabolomics, machine learning

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants

Stipend

Base Stipend Travel Allotment Supplementation
$72,028.00 $3,000.00
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