We are seeking a postdoctoral fellow to conduct research in a new project to create “Proteome in a Bottle” reference samples, leveraging the successful Genome in a Bottle (GIAB) Consortium’s open-consented whole genome reference samples. The goal of the project is to make a suite of ‘omics reference samples whose transcriptome, epigenome, proteome, and metabolomic composition arises from different cell lineages. Such a suite of materials would underpin measurement and studies of the ‘omes, and basic biology.
This project has essential science and technology elements to both develop and characterize the samples. Contemporary stem cell derivation and culturing protocols will be applied to establish high-purity lineages. State-of-the-art mass spectrometry and chemical identification methods will be used to create mass spectral libraries and “body maps” of the proteome from the different lineages.
All of these elements will be applied across multiple preparations and measurement modes to establish repeatability and reproducibility, so reference samples and data can be produced and shared.
The Genome in a Bottle sample set selected from the Personal Genomes Project is amongst the best-characterized genomes available, and induced pluripotent stem cell lines are available from these individuals. Successful development and characterization of suites of samples may result in new NIST reference materials and reference data.
Research would be conducted collaboratively between the Joint Initiative for Metrology in Biology (JIMB) at Stanford University in California, as well as at NIST in Maryland. This collaboration will combine the strengths of both partners with a focus on their shared interest in measurement science development, innovation, and dissemination.