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Opportunity at National Institute of Standards and Technology (NIST)

Engineering Enzymes for the Biomanufacture of Nucleoside Conjugates

Location

Material Measurement Laboratory, Biomolecular Measurement Division

RO# Location
50.64.51.B7943 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Kelman, Zvi zvi.kelman@nist.gov 240.314.294

Description

Nucleoside analogues are widely used as antiviral and antitumor drugs by blocking the activity of DNA or RNA polymerases. An archaeal enzyme that converts a nucleotide triphosphate (NTP) to a nucleotide monophosphate (NMP)-small molecule (SM) adduct via formation of a NMP-O-R or a NMP-N-R linkage was recently identified in the laboratory. While the biological significance of this activity is unknown, its utility as a potentially inexpensive and bio-friendly method for biomanufacturing nucleoside analog “pro-drugs” is being explored. Ongoing research focuses on determining the efficiency and selectivity of the enzyme reaction by exploring the chemical space of SMs (i.e., sugars, lipids, amino acids) as well as nucleoside analogues (i.e., ddNTPs) that can used by the enzyme as substrates to generate NMP-SM.

 

References

Galal WC, Pan M, Kelman Z, Hurwitz J: Journal of Biological Chemistry 287: 16209, 2012

Galal WC, et al: Journal of Biological Chemistry 287: 16220, 2012

 

Keywords:
Antiviral drugs; Anticancer drugs; Nucleoside analogues; Primase;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants
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