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Opportunity at National Institute of Standards and Technology (NIST)

Mapping Reponses in Complex Microbial Systems using Quantitative, Hyperspectral Raman Imaging

Location

Material Measurement Laboratory, Biosystems and Biomaterials Division

RO# Location
50.64.41.B8452 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Camp, Charles Henry charles.camp@nist.gov 301.975.4631
Cicerone, Marcus T. marcus.cicerone@nist.gov 301.975.8104
Dunkers, Joy P. joy.dunkers@nist.gov 301.975.6841

Description

Validating and quantifying composition and function of complex microbial systems is a key to progress in elucidating the important role that bacteria play in human health, from the gut microbiome to the global problem of anti-microbial resistance. Traditional characterization approaches are often destructive, limiting in the amount of information they provide, and use exogenous tagging molecules that perturb the system. Quantitative, label-free, in situ characterization methods are essential for gaining insight into bacteria communities in their unperturbed state.

We have developed a broadband coherent anti-Stokes Raman scattering (BCARS) microscope that interrogates the vibrational fingerprint region of intra- and extra-cellular components. Previous work on eukaryotes has demonstrated that subcellular components (DNA, lipids, collagen) and tissue type (normal vs. cancerous) can be discriminated. This project will use BCARS microscopy to perform quantitative characterization of bacteria identity and location, metabolic state, and signaling molecules in clinically relevant mixed microbial communities and biofilms and compare the findings to traditional microbial analytical tools.

 

Reference

Camp CH Jr, et al: “High-speed coherent Raman fingerprint imaging of biological tissues.” Nature Photonics 8: 627-634, 2014

 

Keywords:
Raman spectroscopy; CARS; Spectroscopy; Chemical imaging; Bioimaging; Bacteria; Biofilms; Microbes;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants
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