Opportunity at National Institute of Standards and Technology (NIST)
Advanced Tools to Measure Dynamics of Pluripotency and Differentiation
Material Measurement Laboratory, Biosystems and Biomaterials Division
Please note: This Agency only participates in the February and August reviews.
|Elliott, John Timothy
Induced pluripotent stem cell technologies are a powerful new tool for biomedical research and have the potential to revolutionize medicine. In order to provide a more complete picture of pluripotency and differentiation, data describing the changes in gene expression at the single cell level are needed. Such high resolution data will provide a better mechanistic description of pluripotency and can be used to develop predictive models for differentiation processes. In this project, quantitative live cell imaging and image analysis will be used to follow gene expression dynamics in single cells. Advanced imaging techniques such as by fluorescence, transmitted light, quantitative phase, or surface plasmon resonance microscopy may be used to acquire images from iPS cell cultures. This project may also include the development and testing of new gene reporter iPS cell lines using advanced gene editing technologies.
Bhadriraju K, et al: “Large-scale time-lapse microscopy of Oct4 expression in human embryonic stem cell colonies.” Stem Cell Research (17): 122-129, 2016
Bioengineering; Quantitative biology; Stem cell; Pluripotency; Differentiation; Microscopy; Image analysis; Biological noise; Gene editing;
Open to U.S. citizens
Open to Postdoctoral applicants