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Opportunity at National Institute of Standards and Technology (NIST)

Design Principles for Advanced Pluripotent Stem Cell Culture

Location

Material Measurement Laboratory, Biosystems and Biomaterials Division

RO# Location
50.64.41.B8383 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Elliott, John Timothy jelliott@nist.gov 301.975.8551
Halter, Michael michael.halter@nist.gov 301.461.0956

Description

Induced pluripotent stems cells have the potential to revolutionize medicine. A critical component to the translation of these cells into therapeutic applications is design principles for predictably and reproducibly culturing cells and efficiently differentiating them into cell types of interest. Typical culture conditions today result in colonies with heterogeneous shapes, sizes, and levels of gene expression. This project focuses on systematically controlling inputs such as shape, size, and spatial distribution of colonies using cell patterning. Techniques such as quantitative fluorescence microscopy, time lapse imaging, image analysis, gene reporter cell lines, and flow cytometry will be used to monitor culture heterogeneity, consistency, and the efficiency of differentiation under highly controlled conditions.

 

References

Bhadriraju K, et al: “Large-scale time-lapse microscopy of Oct4 expression in human embryonic stem cell colonies.” Stem Cell Research 17: 122-129, 2016

Halter M, et al: “Automated live cell imaging of green fluorescent protein degradation in individual fibroblasts.” Cytometry A 71(10): 827-834, 2007

 

Keywords:
Quantitative biology; Stem cell; Pluripotency; Differentiation; Microscopy; Image analysis; Biological noise; Cell culture;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants
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