Opportunity at National Institute of Standards and Technology (NIST)
Tracking Markers of Pluripotency and Differentiation in Live Cells
Material Measurement Laboratory, Biosystems and Biomaterials Division
Please note: This Agency only participates in the February and August reviews.
Time-lapse microscopy of living cells allows the quantification of changes in gene promoter activity by following the intensity of fluorescent proteins in individual cells over time. We have shown that such data can provide information about epigenetic inheritance and that fluctuations in promoter activity can be used to predict rates of state change in cell populations. Applying these techniques to induced pluripotent stem cell (iPSC) colonies will provide a better understanding of mechanisms and efficiency of differentiation, and will allow systematic quantitative assessment of how niche conditions, such as extracellular matrix and influence differentiation. Research challenges include creating iPSC lines with multiple reporter constructs, imaging healthy cells for long times, image analysis, and modeling.
Quantitative biology; Stem cell; Pluripotency; Differentiation; Microscopy; Image analysis; Biological variation; Predictive models;
Open to U.S. citizens
Open to Postdoctoral applicants