Opportunity at National Institute of Standards and Technology (NIST)
Coherent Raman Imaging of Biomineralization Processes in Stem Cells
Material Measurement Laboratory, Biosystems and Biomaterials Division
Please note: This Agency only participates in the February and August reviews.
|Camp, Charles Henry
|Lee, Young Jong
Our goal is to develop methods for label-free chemical imaging and characterization of the biomineralization process. Biomineralization, a process that begins at the subcellular level, organizes to form complex hierarchical structures, eventually forming hard tissues, such as bone and teeth. Biomineralization in vitro is commonly used as a marker to identify stem cell populations, however, there is a lack of understanding regarding the in vitro mineralization process and its relevance to in vivo bone forming capability. Our advanced chemical imaging technique, broadband coherent anti-Stokes Raman scattering (CARS) microscopy, combined with 3D confocal imaging is expected to offer new insights into the mechanisms of cell mediated biomineralization processes. The research opportunity aims to acquire chemical images of key functional markers (e.g., hydroxyapatite, amorphous calcium phosphate, collagen) in cells and tissues without labeling and staining and identify key mineralization features that are relevant for identifying potent stem cell populations. Both stem cells and terminally differentiated cells will be used as model systems. Sensitivity and specificity of the functional markers, as well as other subcellular organelles (e.g., nucleus, mitochondria, cytoskeletons) will be improved through instrumentation optimization and development of robust data analysis procedures.
Biomineralization; CARS microscopy; Bioimaging; Stem cells; Osteoblast; Bone; Raman; Tissue engineering;
Open to U.S. citizens
Open to Postdoctoral applicants