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Opportunity at National Institute of Standards and Technology (NIST)

In-vivo Coherent Raman Imaging of Biomineralization Process

Location

Material Measurement Laboratory, Biosystems and Biomaterials Division

RO# Location
50.64.41.B7848 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Lee, Young Jong youngjong.lee@nist.gov 301.975.6832
Lin-Gibson, Sheng slgibson@nist.gov 301.975.6765

Description

Our goal is to develop methods for label-free chemical imaging and characterization of the calcium phosphate biomineralization process. Biomineralization, a process that begins at the subcellular level, organizes to form complex hierarchical structures, eventually forming hard tissues, such as bone and teeth. Our new chemical imaging technique, broadband coherent anti-Stokes Raman scattering (CARS) microscopy, is expected to offer new insights into the mechanisms of the initial cell mediated processes. The research opportunity aims to acquire in-vivo images of key functional markers (e.g., hydroxyapatite, amorphous calcium phosphate) in cells and tissues without labeling and staining. Both stem cells and terminally differentiated cells will be used as model systems. Sensitivity and specificity of the functional markers, as well as other subcellular organelles (e.g., nucleus, mitochondria, cytoskeletons) will be improved through instrumentation optimization and development of robust data analysis procedures.

 

References

Lee YJ, et al: ACS Macro Letters 1: 1347, 2012

Alfonso PV, Lee YJ, et al: Developmental Cell 22: 1079, 2012

 

Keywords:
Biomineralization; CARS microscopy; Bioimaging; Stem cells; Osteoblast; Bone; Raman; Tissue engineering;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants
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