Opportunity at National Institute of Standards and Technology (NIST)
In-vivo Coherent Raman Imaging of Biomineralization Process
Material Measurement Laboratory, Biosystems and Biomaterials Division
Please note: This Agency only participates in the February and August reviews.
|Lee, Young Jong
Our goal is to develop methods for label-free chemical imaging and characterization of the calcium phosphate biomineralization process. Biomineralization, a process that begins at the subcellular level, organizes to form complex hierarchical structures, eventually forming hard tissues, such as bone and teeth. Our new chemical imaging technique, broadband coherent anti-Stokes Raman scattering (CARS) microscopy, is expected to offer new insights into the mechanisms of the initial cell mediated processes. The research opportunity aims to acquire in-vivo images of key functional markers (e.g., hydroxyapatite, amorphous calcium phosphate) in cells and tissues without labeling and staining. Both stem cells and terminally differentiated cells will be used as model systems. Sensitivity and specificity of the functional markers, as well as other subcellular organelles (e.g., nucleus, mitochondria, cytoskeletons) will be improved through instrumentation optimization and development of robust data analysis procedures.
Lee YJ, et al: ACS Macro Letters 1: 1347, 2012
Alfonso PV, Lee YJ, et al: Developmental Cell 22: 1079, 2012
Biomineralization; CARS microscopy; Bioimaging; Stem cells; Osteoblast; Bone; Raman; Tissue engineering;
Open to U.S. citizens
Open to Postdoctoral applicants