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RAP Lab Opportunities at NIST

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Opportunity at National Institute of Standards and Technology (NIST)

Development of Measurement Technology for Multiplexed Protein Measurements from Single Cells

Location

Material Measurement Laboratory, Biosystems and Biomaterials Division

RO# Location
50.64.41.B7574 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Forry, Samuel P. sam.forry@nist.gov 301.975.5246
Munson, Matthew S matt.munson@nist.gov 650.690.6761

Description

Genetically identical cells in culture show a broad distribution of responses to putatively identical conditions. Current measurement technology only supports the measurement of the mean response of these populations, rather than the diverse responses of the individual cells. This lack of measurement technology has hampered the ability of life science researchers to understand the underlying functional gene and protein networks controlling cellular responses. Using microfluidics, we hope to develop and validate measurement technologies capable of quantifying multiple protein levels from multiple single cell samples within a single experimental platform. In addition, we seek to integrate developed protein measurement platforms with microfluidic cell culture platforms and single-cell measurements of gene expression levels.

 

References

Diercks AH, et al: Analytical Biochemistry 386: 30, 2009

Munson MS, et al: Lab on a Chip 9: 2404, 2010

 

Keywords:
Microfluidic; Proteomics; Single-cell measurements;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Regular applicants
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