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RAP Lab Opportunities at NIST

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Opportunity at National Institute of Standards and Technology (NIST)

Using Nucleic Acids for Massively Multiplexed Measurements other Biological Analytes via Next-Generation Sequencing Platforms

Location

Material Measurement Laboratory, Biosystems and Biomaterials Division

RO# Location
50.64.41.B7573 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Munson, Matthew S matt.munson@nist.gov 650.690.6761
Salit, Marc L. msalit@nist.gov 650.350.2338

Description

Many more robust, accurate measurement tools exist for characterizing the sequence and concentration of nucleic acids than exist for other biological analytes. A major contributing factor to this disparity is the relative stability of the analytes themselves. We seek to develop measurement technologies that quantitatively translate the concentrations of proteins and other analytes to the concentrations of synthetic nucleic acids (e.g., quantitative capture of protein targets using aptamers). Work is in progress on the development of: standards to characterize sequencing platforms for taking census of synthetic populations nucleic acids, techniques for using sequencing platforms to direct evolution of new aptamers, and microfluidic platforms for purification and concentration of free nucleic acids from complex samples such as cell lysate. A combination of these developments with the new, commercially available highly multiplexed sequence platforms will enable massively multiplexed measurements of protein concentrations across a wide dynamic range from single cell samples.

 

Keywords:
Sequencing; Synthetic biology Aptamers; Microfluidics; Proteomics;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Regular applicants
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