Microfluidic systems provide many capabilities that can potentially improve cell culture applications. For example, engineered microenvironments can offer enhanced temporal and spatial control over the soluble microenvironment, minimal dilution of secreted molecules, high degrees of parallelization, and integrated analysis functionality. Additionally, parameters that might better allow imitation of in vivo conditions (e.g., gas partial pressures, media composition, cell density, or organization) could be varied and systematically evaluated. Quality control testing might also be integrated into the cell culture system for routine and automated application. However, establishing cell culture reproducibility and assay accuracy remains challenging, particularly in microfluidic systems. Quality control validation metrics could include gene expression (mRNA), protein production, proliferation rates, cell morphology, and cell motility and could be continuous or endpoint determinations. We are interested in comparing and validating microfluidic cell culture systems against conventional bulk cell cultures and establishing quantitative methods for routine comparisons between cultures in different culture systems. We anticipate that this project will improve the design and development of novel microfluidic cell culture systems and help identify key parameters for enabling reproducible microfluidic cell culture. Since this project is highly interdisciplinary, we are seeking applicants from many diverse fields including chemistry, biology, and engineering.