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RAP Lab Opportunities at NIST

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Opportunity at National Institute of Standards and Technology (NIST)

Microfluidic Cell Culture Metrology

Location

Material Measurement Laboratory, Biosystems and Biomaterials Division

RO# Location
50.64.41.B7124 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Cooksey, Gregory Alan gregory.cooksey@nist.gov 301.975.5529
Elliott, John Timothy jelliott@nist.gov 301.975.8551
Forry, Samuel P. sam.forry@nist.gov 301.975.5246
Kralj, Jason G jason.kralj@nist.gov 301.975.4130
Munson, Matthew S matt.munson@nist.gov 650.690.6761
Plant, Anne L. anne.plant@nist.gov 301.975.3124
Ross, David J. david.ross@nist.gov 301.975.2525

Description

Microfluidic systems provide many capabilities that can potentially improve cell culture applications. For example, engineered microenvironments can offer enhanced temporal and spatial control over the soluble microenvironment, minimal dilution of secreted molecules, high degrees of parallelization, and integrated analysis functionality. Additionally, parameters that might better allow imitation of in vivo conditions (e.g., gas partial pressures, media composition, cell density, or organization) could be varied and systematically evaluated. Quality control testing might also be integrated into the cell culture system for routine and automated application. However, establishing cell culture reproducibility and assay accuracy remains challenging, particularly in microfluidic systems. Quality control validation metrics could include gene expression (mRNA), protein production, proliferation rates, cell morphology, and cell motility and could be continuous or endpoint determinations. We are interested in comparing and validating microfluidic cell culture systems against conventional bulk cell cultures and establishing quantitative methods for routine comparisons between cultures in different culture systems. We anticipate that this project will improve the design and development of novel microfluidic cell culture systems and help identify key parameters for enabling reproducible microfluidic cell culture. Since this project is highly interdisciplinary, we are seeking applicants from many diverse fields including chemistry, biology, and engineering.

 

Keywords:
Cell culture; Gene expression; Microenvironment; Microfluidics; mRNA;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Regular applicants
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