Policy and Global Affairs, Fellowships OfficeThe National Academies Logo

RAP Lab Opportunities at NIST

  Sign In Printable View

Opportunity at National Institute of Standards and Technology (NIST)

Design, Synthesis, and Evaluation of Cellular Probes for Quantitative Microscopy of Living Cells

Location

Material Measurement Laboratory, Biosystems and Biomaterials Division

RO# Location
50.64.41.B6976 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Elliott, John Timothy jelliott@nist.gov 301.975.8551

Description

Data collection for systems biology models requires generating quantitative information about cellular events within living cells. The development of molecular probes that are able to target or enter cells, interact with cellular components, and quantitatively report on the status of an intracellular event will be important tools for generating models for complex biological responses. These cellular probes are likely to be multifunctional hybrid structures of small molecules linked to peptides specifically designed to interact with intracellular molecules such as kinases, proteases, and other enzymatic or structural components. The probes may utilize fluorophores, other spectroscopic tags, or nanoparticles as the quantitative reporter. We are interested in maximizing the use of the optical spectrum and the measurement of multiple intracellular parameters simultaneously. This project would involve design and synthesis of probes utilizing modular chemistry concepts to build hybrid molecular structures that report cellular functions. Work will involve bioconjugate and organic chemistry, purification of probes, and performance testing of the probes. Additional project scopes could include cellular toxicity measurements, live cell measurements with automated fluorescence microscopy or flow cytometry, and quantitative model development.

 

Keywords:
Cell reporters; Fluorescence microscopy; Organic synthetic chemistry; Quantitative cell biology;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Regular applicants
Copyright © 2014. National Academy of Sciences. All rights reserved. 500 Fifth St. N.W., Washington, D.C. 20001.
Terms of Use and Privacy Statement.