The NIST Cellular Biometrology project includes two main thrusts: the development of cell culture capabilities in a microfluidic format and the integration of quantitative cellular analysis in the same platform. Microfluidics promise to revolutionize cell culture by providing exquisite control over the cellular microenvironment, including generation of spatial and temporal concentration gradients of soluble factors and dissolved gases (e.g., O2, and CO2) in a multiplexed fashion, allowing efficient assessment of a wide parameter space. Microfluidics can facilitate investigations of small samples, including individual cells and heterogeneous cell populations. Additionally, parallel analysis of different classes of biomolecules from the same cell(s) is of great interest to enable simultaneous genomics, proteomics, and metabolomics studies for application to systems biology, cellular differentiation, and cytotoxicty.
BioMems; Cell-based assay; Cytotoxicity; Microfluidic; Multiplexed measurements; Nanoparticle; Proteomics; Quantitative cell biology; Systems biology;