The use of 3D scaffolds for tissue generation serves as the basis for the field of tissue engineering. Scaffold properties and composition can strongly influence cell behavior. In this project, new methods for measuring stem cell differentiation in 3D scaffolds will be developed. Effects of scaffold topology, mechanical properties, material composition, pore size, or biofunctionalization will be explored. These measurements will enable identification of specific material properties that can be used to guide cell function and direct stem cell differentiation.
Cell differentiation; Stem cell; Tissue engineering; Regenerative medicine; Scaffold; 3D cell culture;