We have recently demonstrated a broadband CARS (coherent anti-Stokes Raman scattering) microscopy method that is capable of rapidly and noninvasively generating sub-micron resolution, three-dimensional chemical maps of materials, as well as biological cells. CARS microscopy uses molecular vibrational resonances for chemical identification of particular chemical species within a sample of interest. We have demonstrated that CARS microscopy provides sufficient information for noninvasive determination of cell phenotype, but data interpretation methods must be developed in order to realize the full potential of this method.
This research opportunity involves further development of broadband CARS microscopy, as well as application to problems in cell differentiation, intracellular signaling pathways, tissue engineering, and in traditional polymer materials science. Cell-related work may include use of our tissue-culture facilities and collaboration with NIH investigators.
CARS microscopy; Cell-materials interactions; Functional imaging; In situ imaging; Structural imaging; Tissue engineering;