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RAP Lab Opportunities at NIST

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Opportunity at National Institute of Standards and Technology (NIST)

Engineering Extracellular Matrix Proteins: Substrates for Controlling Cells and Tissues

Location

Material Measurement Laboratory, Biosystems and Biomaterials Division

RO# Location
50.64.41.B5208 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Forry, Samuel P. sam.forry@nist.gov 301.975.5246
Plant, Anne L. anne.plant@nist.gov 301.975.3124

Description

A complex array of signals are received by living cells from their surrounding environment and these signals direct cells to proliferate, differentiate, migrate, or apoptose. We are exploring new approaches for studying the nature of these signals from the extracellular matrix and how cells process them. One approach includes the use of self-assembled alkanethiol monolayers and soft lithography patterning of cell adhesion proteins to control cell shape and intracellular signal processing and gene expression. Three-dimensional organization of cells and matrix can also be studied with materials developed in our laboratory. Intracellular signaling response of cells to two-dimensional versus three-dimensional growth conditions could be studied with mammary epithelium and with vascular endothelial cells. Approaches to these studies would include the application of immunohistochemistry and quantitative fluorescence microscopy, AFM, molecular biology techniques, and statistic methods such as principle component analysis.

 

Keywords:
Atomic force microscopy; Cell signaling; Extracellular matrix proteins; Lithography (optical) (soft); Tissue engineering;

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Regular applicants
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