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Opportunity at National Institute of Standards and Technology (NIST)

Confident Identification of Metabolites in Biological Samples

Location

Material Measurement Laboratory, Chemical and Biochemical Reference Data Division

RO# Location
50.63.21.C0659 Gaithersburg, MD

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Telu, Kelly kelly.telu@nist.gov 301-975-8997

Description

Metabolomics can provide insight into many cellular processes. Metabolomic profiles of biological fluids (plasma/serum, urine, etc.) or bioprocess samples (mammalian cell lines or cell culture media) can be used to identify markers relevant to human health or biomanufacturing success.  A critical step in the metabolomic analysis of any such biological mixture is the confident identification of its chemical components. The most sensitive and widely employed method for making such identifications involves matching tandem spectra acquired via liquid chromatography tandem mass spectrometry (LC-MS/MS) against reference spectra in a mass spectral library. However, confident identifications can be very challenging when identifying isomers or related compounds that produce similar mass spectra and separating them from the wide range of accompanying spectra that originate from artifacts and background. The goal of this project is to obtain confident metabolite identifications using both computational methods (such as prior probability or creating a validated material-specific mass spectral library) and experimental methods (such as the use of isotopic labeling methods or orthogonal information like ion mobility). 

Applicants are expected to have knowledge of LC-MS/MS. Knowledge of mass spectral libraries would be beneficial.

References: https://doi.org/10.1002/rcm.7475; https://doi.org/10.1002/bit.27661

Keywords:
Liquid Chromatography; Mass Spectrometry; Metabolomics; Biomanufacturing; Bioinformatics

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants

Stipend

Base Stipend Travel Allotment Supplementation
$72,750.00 $3,000.00
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