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Opportunity at National Institute of Standards and Technology (NIST)

Multiplexed Biomolecular Measurements Using DNA Barcode Sequencing

Location

Material Measurement Laboratory, Material Measurement Laboratory Office

RO# Location
50.63.00.C0104 Gaithersburg, MD 20899

Please note: This Agency only participates in the February and August reviews.

Advisers

Name E-mail Phone
Levy, Sasha Frederic sasha.levy@nist.gov 646-417-3262

Description

Quantitative characterization of the state of biological systems requires simultaneous measurement of many properties. Our group is working to establish DNA barcode-sequencing (bar-seq) as a measurement platform for multiplexed biomolecular measurements, while also working to develop the standards and measurement assurance approaches needed to have confidence in those measurements. We focus on genome-wide and combinatorial measurements such as protein-protein interactions, gene-gene interactions, and RNA-protein interactions using stably integrated engineered DNA barcodes, pooled growth, and next generation sequencing as our readout. One major focus is technology with an emphasis on extension of bar-seq platforms to be higher-throughput and to function in new model systems, and to apply bar-seq to new biological measurements. A second major focus is on bar-seq dissemination with an emphasis on the development of standard barcode libraries and primer sets, robust data analysis pipelines, and the generation of spike-in controls.

Keywords:
bar-seq; sequencing; NGS; barcode; DNA; biology; technology; screening; high-throughput; interaction

Eligibility

Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants
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