Radiation countermeasures are needed to safeguard personnel from both acute, early injury, as well as late-arising pathologies caused by exposure to ionizing radiation. Development of radiation countermeasures will help military personnel, civilians, and first responders in the event of nuclear attacks, as well as improvised nuclear device (IND) or dirty bombs detonation. Such agents can also help cancer patients undergoing radiotherapy. While several radiation countermeasures are in different stages of development, only granulocyte colony-stimulating factor (G-CSF) and PEGylated G-CSF have been approved by the US Food and Drug administration (FDA) as radiomitigators for hematopoietic acute radiation syndrome (H-ARS). There is no radioprotector approved by the FDA. Several agents have been granted investigational new drug (IND) status for acute radiation syndrome by the FDA. We have few potential radiation countermeasures that are under advanced development. We use large and small animal models for developing countermeasures for FDA approval. The goal or our laboratory is to elucidate the mechanism of action of radiation countermeasures at the cellular and molecular levels and to identify and validate biomarkers for the countermeasure efficacy and radiation injury. We use various cellular and molecular biology techniques for investigating different arms of the immune response and signal transduction pathways. We regularly use multiplex Luminex, Western blot analysis, and QRT-PCR for identifying and validating biomarkers. Flow cytometry is used for analyzing various cell surface markers, apoptosis, and progenitor cells. We collaborate with several radiation biologists in the US and other countries to achieve above goals. The laboratory is well-funded with research grants from various agencies of the Department of Defense (CDMRP, DMRDP and JPC7) and Health and Human Services (BARDA and NIAID) in addition to institutional support.
Singh, VK: Scientific Reports 7: 9777, 2017
Singh, VK: International Journal of Radiation and Biololgy 9: 851-869, 2017
Singh, VK: Science Translational Medicine 9: eaal2408, 2017
Singh, VK: Radiation Research 185: 285-298, 2016
Singh, VK: PLoS One 10: e0135388, 2015
Cytokines; Gamma irradiation; Gene expression; Growth factors; Hematopoiesis; Immune response; Progenitor cells; Radioprotectors; Signal transduction pathways;