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Opportunity at Chemical and Biological Defense Funded Laboratories (CBD)

Environmental Detection of Chemical Warfare Agents and Residues Using Engineered Bacteria Incorporating Synthetic Gene Circuits


ECBC-Edgewood Chemical Biological Center, Life Sciences, Medical Sciences, & Behavioral Sciences

RO# Location
01.15.01.B8550 Aberdeen Proving Ground, MD 210105423


Name E-mail Phone
Gibbons, Henry S 410.436.5999


An opportunity is available in the Biosciences Division at the US Army Edgewood Chemical Biological Center to participate in

the development of whole-cell bacterial biosensors for organophosphate-based chemical warfare agents (CWAs). Our laboratory

will be using a combination of classical genetic techniques and high-throughput directed evolution to discover and optimize

genetic elements that confer CWA-dependent expression of reporter genes (e.g. expression of green fluorescent protein or

antibiotic resistance). The successful applicant will develop plasmid-based selectable reporter systems, screen metagenomic DNA

libraries and transposon mutant banks for responsive genetic elements, and evolve existing pesticide-responsive

promoter/repressor systems (Whangsuk et al., Anal. Biochem. 493: 11-13) to respond to CWAs. Ultimately, we aim to develop

synthetic genetic response elements housed in environmentally stable host strains that retain expression of the reporter following

transient exposure to nerve agent that can be detected from a distance (Belkin et al., Nat Biotechnol 35:308-31 0).

Chemical warfare; Organophosphate; Bacterial genetics; Synthetic biology; Biosensor; Detection; Bioprospecting; Metagenomics; Microbiology


Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants
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