Opportunity at Chemical and Biological Defense Funded Laboratories (CBD)
Elucidation of Virulence Mechanisms and Detection of Pathogens
ECBC-Edgewood Chemical Biological Center, Life Sciences, Medical Sciences, & Behavioral Sciences
||Aberdeen Proving Ground, MD 210105423
|Emanuel, Peter A.
Pathogen detection by immunoassay gives the biodefense community an established method for assaying environmental samples. However, this technology has limitations that necessitate the development of additional identification methodologies. Detection by polymerase chain reaction (PCR) amplification of nucleic acids offers an additional level of detection that will increase the capability to differentiate between closely related threats and will augment existing technology for even greater confidence in the identification of biowarfare targets. Real-time PCR detection technologies still have several hurdles that must be overcome before this technology can be fielded to support the military. Research interests include but are not limited to mechanisms of invasion, secretory pathways, micro-RNA characterization, and protein and genetic markers of exposure. Research programs exist for developing new PCR assays for detection of pathogens, lyophilization of completed assays, field testing of assay technology, and the utilization of these assays to elucidate virulence mechanisms.
Polymerase chain reaction; Biodetection; Virulence; Pathogen; Taqman;
Open to U.S. citizens
Open to Postdoctoral applicants